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Measurements of triglyceride are used in the diagnosis and treatment of patients with diabetes mellitus, nephrosis, liver obstruction, other diseases involving lipid metabolism, or various endocrine disorders.
Clinically, triglyceride assays are used to help classify the various genetic and metabolic lipoprotein disorders, and in the assessment of risk factors for atherosclerosis and coronary artery disease.
Triglyceride procedure is based on a series of coupled enzymatic reactions. The triglycerides in the sample are hydrolyzed by a combination of microbial lipases to give glycerol and fatty acids. The glycerol is phosphorylated by adenosine triphosphate (ATP) in the presence of glycerol kinase (GK) to produce glycerol-3-phosphate. The glycerol-3-phosphate is oxidized by molecular oxygen in the presence of GPO (glycerol phosphate oxidase) to produce hydrogen peroxide (H2O2) and dihydroxyacetone phosphate. The formed H2O2 reacts with 4-aminophenazone and N,N-bis(4-sulfobutyl)-3,5-dimethylaniline, disodium salt(MADB) in the presence of peroxidase (POD) to produce a chromophore, which is read at 660/800 nm. The increase in absorbance at 660/800 nm is proportional to the triglyceride content of the sample.
Collect: Serum Separator Tube (SST) - 0.5 ml serum.
Specimen preparation: Serum free from hemolysis is the recommended specimens. Allow blood samples to clot (15 mins). Separate the serum from the cells by centrifuging for 10 minutes. Store serum at 2-8°C until analysis.
Stability: Serum triglyceride is stable for seven days when stored at 2-8°C and 3 months when stored frozen at <-20°C.
At Accu Reference: Refrigerated -7 days: Frozen- 1 monthNOTE: * This test is approved for all states. *
Accu Reference Medical Laboratory
|Cross Reference:||Trig (Triglycerides,Serum)|
< 150 mg/dL Normal
150-199 mg/dL Borderline High
200-499 mg/dL High
> 500 mg/dL Very High
The assay Reportable Range is from 10.0 to 1000 mg/dL. Samples exceeding the upper limit of linearity are diluted and repeated.