Laboratory Test DirectoryShare
Measurements of Total Protein are used in the diagnosis and treatment of a variety of diseases involving the liver, kidney, or bone marrow as well as other metabolic and nutritional disorders.
The total serum protein is the sum of all circulating proteins and is a major component of blood. It is often useful, however, in interpreting the significance of the total protein concentration to have more specific knowledge of individual fractions such as albumins and globulins.
Total Protein procedure is based on the modification of Weichselbaum.2 Cupric ions in an alkaline solution react with proteins and polypeptides containing at least two peptide bonds to produce a violet colored complex. The absorbance of the complex at 540/660 nm is directly proportional to the concentration of protein in the sample.
Collect: Serum Separator Tube (SST) - 0.5 ml serum.
Specimen preparation: Serum free from hemolysis is the recommended specimens. Allow blood samples to clot (15 mins). Separate the serum from the cells by centrifuging for 10 minutes. Store serum at 2-8°C until analysis.
Stability: Protein is stable in serum for one week at room temperature (18 - 25°C) and for one month refrigerated (2 - 8°C).
NOTE: * This test is approved for all states. *
Accu Reference Medical Laboratory
|Cross Reference:||Total Protein (Protein, Total, Plasma or Serum)|
Adults: 6.6 - 8.2 g/dL
The assay Reportable Range is from 3.0 to 12.0 g/dL. Samples exceeding the upper limit of linearity are diluted and repeated.