Laboratory Test DirectoryShare
Iron (non-heme) measurements are used in the diagnosis and treatment of diseases such as iron deficiency anemia, hemochromatosis, and chronic renal disease. Hemochromatosis is a disease associated with widespread deposit in the tissues of two iron containing pigments, hemosiderin and hemofuscin, and characterized by pigmentation of the skin. Transferrin is the major iron carrying protein in the serum.
Iron method utilizes a variation of these methods using TPTZ [2,4,6-Tri-(2-pyridyl)-5-triazine] as the chromogen. In an acid medium, transferrin-bound iron dissociates into free ferric ions and apo-transferrin. Hydrochloric acid and sodium ascorbate reduce the ferric ions to the ferrous state. The ferrous ions then react with TPTZ to form a blue colored complex, which can be measured bichromatically at 600/800 nm. The increase in absorbance is directly proportional to the amount of transferrin bound iron present.
Collect: Serum Separator Tube (SST) - 0.5 ml
Specimen preparation: Serum free from hemolysis is the recommended specimens. Allow blood samples to clot (15 mins). Separate the serum from the cells by centrifuging for 10 minutes. Store serum at 2-8°C until analysis.
Stability: Serum iron is stable for 7 days when stored at 2 - 8°C or 4 days at room temperature (18 - 25°C) after the serum is separated from red cells.
NOTE: * This test is approved for all states. *
Accu Reference Medical Laboratory
Males: 50 - 175 µg/dL
Females: 40 – 175 µg/dL
The assay Reportable Range is from 10.0 to 1000 ug/dL. Samples exceeding the upper limit of linearity are diluted and repeated.