Laboratory Test DirectoryShare
Culture Bordetella pertussis
|Methodology:||Standard reference procedures for Bordetella pertussis culture and identification|
|Reported:||Within 8 days Preliminary: as soon as positive detected; Final: Negative at 8 days|
Collect: Nasal aspirate OR Washing OR Nasopharyngeal Swab.
Specimen Preparation: Aspirate or Washing: Transfer to a sterile container.(Min: 0.5 mL).
Nasopharyngeal Swab: Place swab in Regan-Lowe transport media and incubate for 24-48 hours.
Swab in Jones Kendrick media or Amies with charcoal media, or swabs in described media sent directly without incubation.
Storage/Transport Temperature: Room Temperature.
Unacceptable Conditions: Sputum, Eswabs or medias other than listed above.
Specimen source preferred.
Stability: Incubated Regan-Lowe: Ambient: 4 days; Refrigerated: 4 days; Frozen: Unacceptable
Unincubated Regan-Lowe, Amie with charcoal swab, Aspirate or Washing: Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable