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CPK (Creatinine Phosphokinase)

110

Measurements of Creatine Kinase are used in the diagnosis and treatment of myocardial infarction and muscle disease, such as progressive Duchenne-type muscular dystrophy.
Creatine Kinase (CK) is an enzyme that is found primarily in skeletal muscle, cardiac muscle and brain tissue. Elevated levels of CK are associated with myocardial infarction and various muscle disorders. In myocardial infarction, peak CK levels occur 24 to 36 hours after onset of chest pain and depending on the extent of damage can reach more than 10 times normal levels. 

CK reversibly catalyzes the transfer of a phosphate group from creatine phosphate to adenosine diphosphate (ADP) to give creatine and adenosine triphosphate (ATP) as products. The ATP formed is used to produce glucose-6-phosphate and ADP from glucose. This reaction is catalyzed by hexokinase (HK), which requires magnesium ions for maximum activity. The glucose-6-phosphate is oxidized by the action of the enzyme glucose-6-phosphate dehydrogenase (G6P-DH) with simultaneous reduction of the coenzyme nicotinamide adenine dinucleotide (NADP) to give NADPH and 6-phosphogluconate. The rate of increase in absorbance at 340/660 nm due to the formation of NADPH is directly proportional to the activity of CK in the sample.


Methodology: Quantitative Enzymatic/Electrophoresis
Performed: Sun-Sat
Reported: 1-2 days
Specimen Required: Specimen requirement
Type: SST TUBE 
Specimen preparation: Serum free from hemolysis is the recommended specimens. Allow blood samples to clot (15 mins). Separate the serum from the cells by centrifuging for 10 minutes. Store serum at 2-8°C until analysis. 
Storage/Transport: REFRIGERATED 
Stability: Protect samples from light for maximum stability. CK is stable in serum for up to 4 hours at room temperature (18 - 25°C), 8 - 12 hours refrigerated (2 - 8°C) or up to 1 month at <-20°C.

NOTE: * This test is approved for all states. *

Performing Lab
Accu Reference Medical Laboratory
CPT Code(s): 82552; 82550
Cross Reference: CPK (Creatine Phosphokinase), CK

Reference Range:
    Adults:   23 - 223 U/L

Reportable Range:
The assay Reportable Range is from 10.0 to 2000.0 U/L. Samples exceeding the upper limit of linearity are diluted and repeated. 

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