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The determination of electrolytes (sodium, potassium, and chloride) is one of the most important functions in the clinical laboratory.
Electrolytes affect most metabolic processes. They serve to maintain osmotic pressure and hydration of various body fluid compartments, proper body pH, and regulation of appropriate heart and muscle functions. Electrolytes are also involved in oxidation-reduction reactions and participate as essential parts or cofactors in enzyme reactions.
ISE module for Na+, K+, and Cl- employs crown ether membrane electrodes for sodium and potassium; and a molecular oriented PVC membrane for chloride that are specific for each ion of interest in the sample. An electrical potential is developed according to the Nernst Equation for a specific ion. When compared to the Internal Reference Solution, this electrical potential is translated into voltage and then into the ion concentration of the sample.
|Methodology:||Quantitative Ion-Selective Electrode|
Type: SST TUBE
Specimen preparation: Serum free from hemolysis is the recommended specimens. Allow blood samples to clot (15 mins). Separate the serum from the cells by centrifuging for 10 minutes. Store serum at 2-8°C until analysis.
Unacceptable Conditions: Body Fluid, Urine, Grossly hemolyzed.
Stability: Use fresh sample for analysis when possible. Sodium and potassium are stable in serum for at least one week when stored at 2-8°C. Chloride is stable in serum for one week when stored 2-30°C. Store the samples in a stoppered tube if analysis is delayed.
Accu Reference Medical Laboratory