Laboratory Test DirectoryShare
Chlamydia trachomatis/N. gonorrhoeae rRNA PLUS (TMA)
|Methodology:||Thermomechanical analysis (TMA)|
Collect: Gen-Probe (Aptima) Swab, Gen-Probe (Apt) Vag Swab (GenProbeVa), Genprobe PACE Swab (GenProbe), M4 Transport Media/Swab (M4), SurePath Vial, ThinPrep Vial, Universal Transport Media (UTM Sterile).
Specimen Preparation: Swabs with wooden shafts are NOT acceptable.
1. Endocervical swab specimens (Genprobe): Remove excess mucus from cervical os and surrounding mucosa using cleaning swab (white shaft). Discard swab. Insert specimen collection swab (blue shaft) into endocervical canal. Gently rotate rotate swab clockwise for 10-30 seconds in the endoverical canal to to ensure adequate sampling. Withdraw swab carefully; avoid any contact with vaginal mucosa. Immediately place specimen collection swab into transport tube. Carefully break swab shaft at scoreline; use care to avoid splashing of contents. Re-cap swab specimen transport tube tightly.
2. Male urethral swab specimens (Genprobe): Patient should not have urinated for at least one hour prior to sample collection. Insert specimen collection swab (blue shaft) 2-4 cm into urethra. Gently rotate swab clockwise for 2-3 seconds in urethra to ensure adequate sampling. Withdraw swab carefully. Immediately place specimen collection swab into transport tube. Carefully break swab shaft at scoreline; use care to avoid splashing of contents. Re-cap swab specimen transport tube tightly. Collection instructions for PACE samples: Specimens are collected according to the appropriate instructions supplied with the PACE Specimen Collection Kits for Endocervical and Urethral Specimens.
Specimens in alternate transport media (M4, ThinPrep, SurePath, culturette swabs) have been validated by Quest Diagnostics Nichols Institute.
Rejection Criteria: BD ProbeTec Vials
NOTE: ADDITIONAL COLLECTION INSTRUCTIONS:
ThinPrep Pap Test Specimens: Specimens collected using broom-type device and placed in Cytyc PreservCyt Solution for use in making ThinPrep Pap Test slides can be used for CT/GC testing. Collect specimens in routine manner
APTIMA Vaginal swab specimens (both clinician-collected and patient-collected):
1. Partially peel open the swab package. Do not touch the soft tip or lay the swab down. Remove the swab. Hold the swab, placing the thumb and forefinger in the middle of the swab shaft.
2. Carefully insert the swab into the vagina about two inches past the introitus and gently rotate the swab for 10 to 30 seconds. Make sure the swab touches the walls of the vagina so that moisture is absrobed by the swab.
3. Withdraw the swab without touching the skin.
4. While holding the swab in the same hand, unscrew the cap from the tube. Immediately place the swab into the transport tube so that the tip of the swab is visible below the tube label.
5. Carefully break the swab shaft against the side of the tube. Tightly screw the cap onto the tube.
NOTES: Due to the sensitivity of the APTIMA TMA assay, reflex testing of samples previously assayed by Gen-Probe PACE is not recommended.
Storage/Transport Temperature: Room Temperature
Stability: Gen-Probe (Apt) Vag Swab (GenProbeVa) : Room Temperature/Refrigerated - 60 Days;
Genprobe PACE Swab (GenProbe) : Refrigerated/Room Temperature - 7 Days;
M4 Transport Media/Swab (M4): Room Temperature/Refrigerated - 4 days;
SurePath Vial : Refrigerated- 6 days, Room Temperature - 28 days;
ThinPrep Vial : Room Temperature/Refrigerated - 21 days. Use ThinPrep Pap Test Collection Kit and endocervical Broom/Brush///collection device.
Universal Transport Media: Refrigerated/Room Temperature - 4 days
Note: This test is approved for NY .
Quest Diagnostics Nichols INST
|Test Panel Components||Neisseria gonorrhoeae rRNA ; Chlamydia trachomatis rRNA.|