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Bicarbonate measurements are used in the diagnosis and treatment of numerous potentially serious disorders associated with changes in body acid-base balance. The determination of bicarbonate (HCO3¯) is used in conjunction with other clinical and laboratory information for the evaluation of acid-base status. An elevation of the Bicarbonate level may be observed in compensated respiratory acidosis and metabolic alkalosis. Low Bicarbonate levels may be observed in compensated respiratory alkalosis and metabolic acidosis.1 Additional laboratory determinations will permit
differentiation between metabolic and respiratory conditions.
The bicarbonate reagent utilizes the enzymatic. In this procedure bicarbonate (HCO ¯) and phosphoenolpyruvate (PEP) are converted to oxaloacetate and phosphate in the reaction catalyzed by phosphoenolpyruvate carboxylase (PEPC). Malate dehydrogenase (MD) catalyzes the reduction of oxaloacetate to malate with the concomitant oxidation of reduced nicotinamide adenine dinucleotide (NADH). This oxidation of NADH results in a decrease in absorbance of the reaction mixture measured bichromatically at 380/410 nm proportional to the Bicarbonate content of samples.
Collect: Serum Separator Tube (SST) - 0.5 ml
Specimen preparation: Serum free from hemolysis is the recommended specimens. Allow blood samples to clot (15 mins). Separate the serum from the cells by centrifuging for 10 minutes. Store serum at 2-8°C until analysis.
Stability: Once separated from cells, Bicarbonate in serum is stable for 24 hours when stored at 20 – 25°C and protected from exposure to air.
Accu Reference Medical Laboratory
Adults: 17 - 32 mEq/L
The assay Reportable Range is from 2.0 to 45.0 mEq/L. Samples exceeding the upper limit of linearity are diluted and repeated.