Laboratory Test DirectoryShare
Measurement of the levels of bilirubin, an organic compound formed during the normal and abnormal destruction of red blood cells, is used in the diagnosis and treatment of liver, hemolytic, hematologic, and metabolic disorders, including hepatitis and gall bladder block.
Total Bilirubin Reagent is a variation of the classical method. A stabilized diazonium salt, 3,5-dichlorophenyl-diazonium tetrafluoroborate (DPD), reacts with bilirubin to form azobilirubin which absorbs maximally at 570/660 nm. Caffeine and a surfactant are used as reaction accelerators.
The absorbance at 570/660 nm is proportional to the bilirubin concentration in the sample. A separate serum blank is performed to eliminate endogenous serum interferences.
Collect: Serum Separator Tube (SST) - 0.5 ml
Specimen preparation: Serum free from hemolysis is the recommended specimens. Allow blood samples to clot (15 mins). Separate the serum from the cells by centrifuging for 10 minutes. Store serum at 2-8°C until analysis.
Stability: It has been reported that exposure to direct sunlight can decrease bilirubin in samples by 50% within one hour. When well protected from light, bilirubin in serum is stable for 3 days when stored at 2 - 8°C, or three months when stored at <-20°C.
At Accu Reference: Refrigerated - 3 days: Frozen- 1 month
NOTE: * This test is approved for all states. *
Accu Reference Medical Laboratory
Adults : 0.2 - 1.0 mg/dL
The assay Reportable Range is from 0.0 to 30.0 mg/dL. Samples exceeding the upper limit of linearity are diluted and repeated.