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QuantiFERON-TB Gold In-Tube, 3 tube kits
|Performed:||Monday - Saturday|
Collect: #1) QFT-NIL Control Tube WB 1 mL
#2) QFT-TB Antigen Tube WB 1 mL
#3) QFT-Mitogen Control WB 1 mL
Note: Specimen collection locations >3350 feet above sea level should use the QuantiFERON® collection kit designed for "high altitude" (tubes with a gold ring on tube top).
1. For each patient, collect 1 mL of blood by venipuncture directly into each of the three (3) unique QuantiFERON®-TB Gold IT blood collection tubes. Tubes must be at room temperature prior to collection. Under or overfilling of the tubes may lead to erroneous results.
2. Shake them ten (10) times just firmly enough to ensure the entire surface of the tube is coated with blood, to solubilize antigens on tube walls.
3. Incubate the three (3) tubes upright at 36-38° C within 16 hours for 16 to 24 hours. The specimen rack should sit off the floor of the incubator. If the blood is not incubated immediately after collection, remixing of the tubes by inverting 10 times must be repeated immediately prior to incubation.
4. Following incubation either:
A. Immediately transport the three (3) transport tubes to the Laboratory between 4 and 27° C. Samples will be stable for 72 hours at 4-27° C (room temperature or refrigerated). *OR*
B. Centrifuge each of the three (3) incubated collection tubes for 15 minutes at 2000-3000 RCF(G). Label with patient name, identification number, and date of collection. Deliver to the Laboratory at 2-8° C. Samples will be stable for 28 days at 2-8° C (refrigerated).
Storage/Transport: Transport Container QTF-Nil control (gray cap, white or gold ring) tube QFT-TB antigen (red cap, white or gold ring) tube QFT-Mitogen control (purple cap, white or gold ring) tube. Transport Temperature: See Instructions
Stability: Uncentrifuged specimens: Room temperature: 72 hours,Refrigerated: 72 hours, Frozen: Unacceptable
Centrifuged specimens: Room temperature: 72 hours, Refrigerated: 28 days, Frozen: Unacceptable
Reject Criteria: Received >72 hours (room temperature) • Received frozen • Specimens not collected in Quantiferon® Gold IT blood collection tubes
NOTE: * This test is approved for all states. *
Quest Diagnostics NY/NJ