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Many epidemiological investigations have demonstrated the strong and independent inverse association between HDL-Cholesterol and the risk of coronary artery disease. It has been proposed that HDL particles, through the uptake and transport of cholesterol from peripheral tissue to the liver (reverse cholesterol transport), protects against the development of atheromatous plaques.
HDL-Cholesterol test (HDL-C) is a two reagent homogenous system for the selective measurement of serum HDL-Cholesterol in the presence of other lipoprotein particles. The assay is comprised of two distinct phases. In phase one, free cholesterol in non-HDL-lipoproteins is solubilized and consumed by cholesterol oxidase, peroxidase, and DSBmT to generate a colorless end product. In phase two, a unique detergent selectively solubilizes HDL-lipoproteins. The HDL cholesterol is released for reaction with cholesterol esterase, cholesterol oxidase, and a chromogen system to yield a blue color complex, which can be measured bichromatically at 600/700nm. The resulting increase in absorbance is directly proportional to the HDL-C concentration in the sample.
Collect: Serum Separator Tube (SST) - 0.5 ml
Specimen preparation: Serum free from hemolysis is the recommended specimens. Allow blood samples to clot (15 mins). Separate the serum from the cells by centrifuging for 10 minutes. Store serum at 2-8°C until analysis.
Stability: Use fresh sample for analysis when possible. Serum should not remain at room temperature longer than 14 hours. Should analysis not be completed within 14 hours, serum samples may be stored refrigerated for up to one week. If specimens need to be stored for more than 1 week, they may be preserved frozen at -70C for up to 3 months. Avoid repeated freezing and thawing of the sample. Samples should only be frozen once.
Accu Reference Medical Laboratory
|Cross Reference:||Cholesterol (HDL Cholesterol)|
Adults: 40- 199 mg/dL
The NCEP guidelines classify HDL- C levels as follows:
1. < 40 mg/dL as indicative of a major risk factor for Coronary Heart Disease.
2. > 60 mg/dL as a negative risk factor for Coronary Heart Disease.
The assay Reportable Range is from 3 to 200 mg/dL. Samples exceeding the upper limit of linearity are diluted and repeated.